Processing MALDI mass spectra to improve mass spectral direct tissue analysis
نویسندگان
چکیده
منابع مشابه
On-tissue Direct Monitoring of Global Hydrogen/Deuterium Exchange by MALDI Mass Spectrometry: Tissue Deuterium Exchange Mass Spectrometry (TDXMS).
Hydrogen/deuterium exchange mass spectrometric (H/DXMS) methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exchange behavior of proteins in their native microenvironment. Using this method, a differ...
متن کاملDNA sequence analysis by MALDI mass spectrometry.
Conventional DNA sequencing is based on gel electrophoretic separation of the sequencing products. Gel casting and electrophoresis are the time limiting steps, and the gel separation is occasionally imperfect due to aberrant mobility of certain fragments, leading to erroneous sequence determination. Furthermore, illegitimately terminated products frequently cannot be distinguished from correctl...
متن کاملMALDI-TOF mass spectrometry for direct bacterial identification
18 An ammonium chloride erythrocytes-lysing procedure was used to prepare a bacterial pellet 19 from positive blood cultures for direct MALDI-TOF mass spectrometry analysis. 20 Identification was obtained for 78.7% of pellets. Moreover, 99% of MALDI-TOF 21 identifications were congruent at species level when considering valid scores. This fast and 22 accurate method is promising. 23
متن کاملDirect ionization of biological tissue for mass spectrometric analysis.
Analysis of biological tissue at a molecular level is of great importance in biological, medical and clinical studies. In this manuscript, we report that both plant and animal tissues can be directly ionized and analyzed by mass spectrometry under ambient conditions. By adding some solvents and applying a high voltage, spray ionization can be induced at the tip of biological tissue and a mass s...
متن کاملNanostructure initiator mass spectrometry: tissue imaging and direct biofluid analysis.
Nanostructure initiator mass spectrometry (NIMS) is a recently introduced matrix-free desorption/ionization platform that requires minimal sample preparation. Its application to xenobiotics and endogenous metabolites in tissues is demonstrated, where clozapine and N-desmethylclozapine were observed from mouse and rat brain sections. It has also been applied to direct biofluid analysis where ket...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Mass Spectrometry
سال: 2007
ISSN: 1387-3806
DOI: 10.1016/j.ijms.2006.10.005